cell analyzer 2000  (Cytiva Europe)

Journal: Communications Biology

Article Title: Plasmodium berghei liver stage parasites exploit host GABARAP proteins for TFEB activation

doi: 10.1038/s42003-024-07242-x

Figure Lengend Snippet: a P. berghei survival is reduced in TFEB-KO cells. HeLa WT, TFEB-KO, and TFEB-KO + TFEBmCh cells were infected with PbGFP . At 6 and 48 hpi, parasite numbers were evaluated using automated high throughput live cell imaging and analysis (INCell Analyzer 2000). The graph shows relative parasite survival from 6 to 48 hpi compared to the WT control. The mean and SD of three independent experiments are depicted. N > 500 parasites per cell line and experiment. A one-way ANOVA test was used to determine p -values. b Parasite size at 48 hpi of the experiment described in ( a ). The experiment was performed three times. Shown is the data of one representative experiment. Median and SD are depicted. P -values were calculated using a one-way ANOVA test. HeLa WT N = 193; TFEB-KO N = 129; TFEB-KO + TFEBmCh N = 237. c Western blot of the cell lines used in ( a ) and ( b ). Whole protein lysates were separated on a 12% acrylamide gel, transferred onto a nitrocellulose membrane, and probed with an anti-TFEB antibody. α-tubulin was detected as a loading control. Note that in line 3, the TFEBmCh fusion protein expressed in the TFEB-KO cells is larger than the endogenously expressed TFEB due to the mCherry fusion partner (see also Fig. S ).

Article Snippet: The same cells were imaged with an INCell Analyzer 2000 automated live cell imaging system (GE Healthcare Life Sciences) at 6, 24, and 48 hpi with minimal light exposure (15–25 ms).

Techniques: Infection, High Throughput Screening Assay, Live Cell Imaging, Control, Western Blot, Acrylamide Gel Assay, Membrane